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- 产品/服务:HTB-81 DU 145 人前列腺癌细胞销售
- 型 号:HTB-81
- 品 牌:上海复祥生物
- 单 价:面议
- 更新日期:2024-03-23
- 有效期至:长期有效
- 浏览次数:2036
- 立即询价
HTB-81 DU 145 人前列腺癌细胞销售, ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!...
HTB-81 DU 145 人前列腺癌细胞销售
ATCC® Number: HTB-81™
Designations: DU 145
Depositors: KR Stone
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: prostate
Disease: carcinoma
Derived from me
Permits/Forms: In addition to the MTA mentio
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Antigen ex
DNA Profile (STR): D7S820: 7, 10, 11
D13S317: 12, 13, 14
D5S818: 10, 13
vWA: 17, 18, 19
THO1: 7
CSF1PO: 10, 11
TPOX: 11
D16S539: 11, 13
Amelogenin: X, Y
Cytogenetic Analysis: This is a hypotriploid human cell line. Both 61 and 62 chromosome numbers had the highest rate of occurrence in 30 me
HTB-81 DU 145 人前列腺癌细胞销售
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1-2
PGM1, 1
PGM3, 2
Age: 69 years
Gender: male
Ethnicity: Caucasian
Comments: The line is not detectably hormone sensitive, is o
Propagation: ATCC complete growth medium: The ba
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which co
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor temperature
Related Products: Recommended medium (without the additio
recommended serum:ATCC 30-2020
purified DNA:ATCC HTB-81D
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
References: 22289: Papsidero LD, et al. Prostate antigen: a marker for human prostate epithelial cells. J. Natl. Cancer Inst. 66: 37-42, 1981. PubMed: 6935463
22858: Stone KR, et al. Isolation of a human prostate carcinoma cell line (DU 145). Int. J. Cancer 21: 274-281, 1978. PubMed: 631930
23028: Mickey DD, et al. Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice. Cancer Res. 37: 4049-4058, 1977. PubMed: 908039
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen ex
32283: Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene ex
32341: Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer
HTB-81 DU 145 人前列腺癌细胞销售
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